Senior Research Scientist I - Cancer Dependency Map

The Broad Institute's Cancer Program is at the forefront of advancing cancer research and therapeutic discovery. A cornerstone of this program is the Cancer Dependency Map (DepMap), a transformative initiative that systematically maps cancer vulnerabilities using functional genomics, high-throughput screening, and cutting-edge computational tools. Our mission is to identify optimal therapeutic targets and accelerate drug discovery. As part of our continued growth, DepMap is expanding its efforts through strategic collaborations like the DepMap Consortium-a partnership with top pharmaceutical and biotech companies-as well as PedDep, a focused initiative to uncover new treatments for childhood cancers.

We are seeking a highly motivated and innovative Research Scientist with deep expertise in single-cell RNA perturb-seq to join our dynamic and collaborative team. This role offers a unique opportunity to drive the development and application of advanced single-cell technologies in a mission-driven environment committed to transforming cancer treatment.

Key Responsibilities

• Design, execute, and analyze single-cell RNA-sequencing and perturb-seq experiments to uncover cancer vulnerabilities.
• Develop, refine, and implement novel single-cell genomic assays and high-throughput workflows.
• Collaborate closely with computational biologists and data analysts to interpret complex datasets and generate actionable biological insights.
• Mentor and provide scientific leadership to 2+ junior team members, contributing to their technical and professional development.
• Apply single-cell technologies to functionally characterize cancer models and identify novel therapeutic targets.
• Maintain and optimize mammalian cell culture systems for screening experiments.
• Prepare high-quality documentation of protocols, experimental design, and results.
• Present findings to internal stakeholders, external collaborators, and at scientific meetings.
• Support the development and implementation of standard operating procedures (SOPs) to ensure rigor and reproducibility.

Qualifications

• Ph.D. (or equivalent experience) in Molecular Biology, Genomics, Cell Biology, Bioinformatics, or a related life sciences field.
• 2+ years of hands-on experience in single-cell RNA-seq technologies, with a strong foundation in perturb-seq and related methods.
• Experience with CRISPR-based screening approaches is highly desirable.
• Strong quantitative and analytical skills; comfortable working with large biological datasets and integrating experimental with computational analysis.
• Proficient in mammalian cell culture and manipulation techniques.
• Excellent communication and collaboration skills; demonstrated ability to work effectively in cross-functional, fast-paced research teams.
• A passion for innovation, continuous learning, and making a meaningful impact in cancer research.

Why Join Us?

• Contribute to a mission-driven team developing next-generation cancer therapeutics.
• Access cutting-edge resources, including state-of-the-art genomics platforms and long-standing expertise in data generation and analysis.
• Work in a collaborative environment that values scientific excellence, creativity, and impact.
• Engage with top-tier academic, biotech, and pharmaceutical partners through the DepMap Consortium and PedDep.
• Be part of a vibrant, intellectually stimulating scientific community within the Broad Institute, where your work directly contributes to changing the landscape of cancer treatment.